This question ultimately hinges on knowing the difference between ribozymes and spliceosomes because transferase, hydrolase, and lyase should all be recognized as proteins that function as enzymes. Transferase catalyzes reactions that facilitate the transfer of functional groups. Hydrolase works to catalyze hydrolysis reactions. Lyase works to catalyze reactions that break down double bonds. Spliceosomes are a unit of proteins and RNA that work to catalyze reactions that splice out introns in RNA to form mature mRNA ready for translation. Ribozymes are important because they also splice RNA into mRNA, but they do not have a protein component to them. The discovery of Ribozymes was a breakthrough in that it was the first evidence that not all enzymes are proteins.

DNA helicase is an enzyme that is able to slip between the two strands of DNA and disrupt the hydrogen bonds that keep the DNA in the double helix structure. This disruption opens up the DNA helix, and exposes sections of DNA that can then be transcribed or replicated. As helicase moves down the double helix, the DNA reforms into a double helix since the enzyme is no longer blocking the hydrogen bonds.

These are all definitive traits of an enzyme. Enzymes are proteins which are extremely helpful in speeding up certain reactions without being depleted by the reactions themselves (as such, they are catalysts for these reactions). Enzymes reduce the amount of energy needed for a reaction to occur, generally because they facilitate reactions by recognizing reactants and bringing them into contact with each other. This occurs when the reactants bind to certain parts of the enzyme (active sites), which causes the enzyme to change shape and bring the reactants into contact with each other (and then the reactants can bind to form the product).

肽基转移酶的酶在conjunction with tRNA molecules to extend a growing polypeptide chain at the ribosome during translation. Ligase is not used at all in translation, nor is topoisomerase or ATP synthase. tRNA synthetase is used to bind the correct amino acids to corresponding tRNA molecules, but it is not used to extend the polypeptide at the ribosome.

While all the answer choices are important in DNA replication, only DNA Polymerase I performs this particular function. Ligase helps bind the newly replaced DNA nucleotides to the rest of the DNA strand. DNA polymerase III is the main synthesizing enzyme of DNA replication, and creates the majority of the DNA strand. DNA polymerase II is less well known than I and III, but it is believed to perform as a repair enzyme which removes incorrectly paired segments of DNA (which can then be filled back in by DNA polymerase I).