GRE Subject Test: Biochemistry, Cell, and Molecular Biology : Help with Ribosome and tRNA Structure

Study concepts, example questions & explanations for GRE Subject Test: Biochemistry, Cell, and Molecular Biology

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一个ll GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources

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Example Questions

Example Question #21 :Rna, Transcription, And Translation

To which of the following structures in a typical molecule of tRNA is an amino acid attached?

Possible Answers:

D loop

CCA tail

Variable loop

一个nticodon loop

Correct answer:

CCA tail

Explanation:

一个mino acids are attached to the CCA tail of a tRNA. These are found at the 3' end of tRNA molecules and are important for recognition by aminoacyl tRNA synthetases (enzymes that actually attach the amino acids to the tRNA). The anticodon loop, as the name suggests, contains the anticodon, which will be important during translation for recognizing mRNA sequences. The D-loop and the variable loop are other portions of the tRNA that are important for maintaining structure and recognition.

Example Question #1 :Translation And Proteins

Chloramphenicol prevents protein translation by which of the following mechanisms?

Possible Answers:

It blocks the peptidyl transferase reaction on ribosomes

It blocks the binding of aminoacyl tRNA to the A site of the ribosome

It blocks the translocation reaction on ribosomes

It blocks initiation of RNA chains by binding to RNA polymerase

Correct answer:

It blocks the peptidyl transferase reaction on ribosomes

Explanation:

Tetracycline blocks the binding of aminoacyl tRNA to the A site of the ribosome.

Cyclohexamide blocks the translocation reaction on ribosomes.

Rifamycin blocks the initiation of RNA chains by binding to RNA polymerase.

Chloramphenicol blocks the pepidyl transferase reaction on the ribosome.

Example Question #1 :Translation And Proteins

During translation, which site in the ribosome allows for tRNA moelcules to enter the complex?

Possible Answers:

P site

一个site

E site

R site

Correct answer:

一个site

Explanation:

The ribosomal complex has three sites where tRNA moelcules can be oriented during the process of translation: the A site, the P site, and the E site. During polypeptide elongation, a tRNA with an attached amino acid will enter at the A site. It will then move to the P site, now holding the growing polypeptide chain. All tRNAs no longer holding an amino acid will exit the ribosome at the E site.

Example Question #30 :Rna, Transcription, And Translation

On which of the following molecules could you find an anticodon?

Possible Answers:

tRNA

mRNA

miRNA

rRNA

Correct answer:

tRNA

Explanation:

In order to make sure that the proper amino acid is added to the growing polypeptide chain, an anticodon found on the tRNA carrying the amino acid must be a match for the codon found on the mRNA.

Example Question #31 :Rna, Transcription, And Translation

Which of the following most accurately describes the chronological order of ribosome biogenesis in eukaryotes?

Possible Answers:

细胞质中的核糖体蛋白质翻译. At the same time, rRNA genes are being rapidly transcribed in the nucleolus. rRNA is transported to the cytoplasm where the rRNA and ribosomal proteins form the 60S and 40S ribosomal subunits.

Ribosomal proteins are translated in the nucleous and transported to the cytoplasm. At the same time, rRNA genes are being rapidly transcribed in the nucleolus. rRNA and ribosomal proteins form the 60S and 40S subunits in the cytoplasm, where they join to form a functional ribosome.

细胞质中的核糖体蛋白质翻译and transported to the nucleolus. At the same time, rRNA genes are being rapidly transcribed in the cytoplasm. rRNA and ribosomal proteins form the 60S and 40S subunits in the cytoplasm.

细胞质中的核糖体蛋白质翻译and transported to the nucleolus. At the same time, rRNA genes are being rapidly transcribed in the nucleolus. rRNA and ribosomal proteins form the 50S and 30S subunits in the nucleolus and are then transported to the cytoplasm for functional ribosome assembly.

细胞质中的核糖体蛋白质翻译and transported to the nucleolus. At the same time, rRNA genes are being rapidly transcribed in the nucleolus. rRNA and ribosomal proteins form the 60S and 40S subunits in the nucleolus and are then transported to the cytoplasm for functional ribosome assembly.

Correct answer:

细胞质中的核糖体蛋白质翻译and transported to the nucleolus. At the same time, rRNA genes are being rapidly transcribed in the nucleolus. rRNA and ribosomal proteins form the 60S and 40S subunits in the nucleolus and are then transported to the cytoplasm for functional ribosome assembly.

Explanation:

细胞质中的核糖体蛋白质翻译and rRNA genes are transcribed in the nucleolus. Following protein translation, these proteins enter the nucleus through nuclear pores and localize to the nucleolus. Here, transcribed rRNA associates with the ribosomal proteins to form the 60S and 40S eukaryotic ribosomal subunits. Prokaryotes have 50S and 30S subunits. The ribosomal subunits then translocate to the cytoplasm where they join together to form fully functional ribosomes.

Example Question #111 :Molecular Biology And Genetics

How many ribosomal binding sites are there and what are their functions?

Possible Answers:

There are two sites. A site binds free tRNA before ribosomal exit, P site binds peptidyl-tRNA

There are three sites. A site binds aminoacyl-tRNA, P site binds peptidyl-tRNA, E site binds free tRNA before ribosomal exit

There are two sites. A site binds free tRNA before ribosomal exit, P site binds aminoacyl-tRNA

There are three sites. A site binds peptidyl-tRNA, P site binds aminoacyl-tRNA, E site binds free tRNA before ribosomal exit

There are three sites. A site binds free tRNA before ribosomal exit, P site binds aminoacyl-tRNA, E site binds peptidyl-tRNA

Correct answer:

There are three sites. A site binds aminoacyl-tRNA, P site binds peptidyl-tRNA, E site binds free tRNA before ribosomal exit

Explanation:

The correct answer is there are three sites. A site binds aminoacyl-tRNA, P site binds peptidyl-tRNA, E site binds free tRNA before ribosomal exit.

一个ll GRE Subject Test: Biochemistry, Cell, and Molecular Biology Resources

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